The present study was aimed to determine the ameliorative effect of caffeine on
Body weight and lipid profile in male rats treated with hydrogen peroxide (H2O2) .
Seventy Two adult male rats were used in this study . The study included two
experiments ,in each experiments 36 males were randomly assigned two six equal groups
of six animals in each group .The animals in both experiments were treated with the
same substances and doses for each group as follows .Group one (control) animals were
drenched normal saline ,Group two animals were treated (5.63 mg/kg. Bw )H2O2 daily
by oral gavage also group three , animals were treated with low dose caffeine (150 mg
/kg Bw) daily .Group four , animals were treated with high dose caffeine (250 mg /kg
Bw) daily . Group five . animals were treated (5.63 mg/kg. Bw )H2O2 dose after 1 h
animals were given low dose of caffeine (150 mg/kg Bw) .Group Six animal were
treated with H2O2 dose(5.63 mg/kg Bw) each rat after one hour was given high dose of
caffeine (250 mg /Kg Bw) . The first experiments lasted for one month and second
experiments lasted for two months . At the end of the two experiments, animals of all
group were sacrificed under chloroform anesthesia .Blood samples were collected from the heart directly by cardiac puncture and the serum was separated to measure the lipid
profile .
The result revealed a significant decreased in body weight gain in H2O2in first and
second experiments compared with control group . While a significant improvements
were recorded in body weight gain in all treated groups compared with H2O2 group but
still significantly lower compared with those of control group. A significant increase in
TC ,TG and LDL-c were recorded in H2O2 group in both experiments compared with
control group on the other hand no significant difference was recorded in HDL-c level
in H2O2group in first experiments while a significant decreased was recorded in second
experiments compared with control group . Finally a significant degrees of
improvement were observed in lipid profile in all treated group compared with H2O2 .