The functions of  the ovaries are controlled by many   exogenous and endogenous factors,  including changes in the biochemical and endocrine glands that occur in the follicular fluid during the breeding season in the bitches. The aim of this study was  to determine and compare the concentrations some hormonal in the peripheral circulation and follicular fluid of bitches during the breeding season.  For this purpose, ovaries collected from adult bitches immediately after ovariectomy,  and blood samples were  also collected from these bitches before and after season. The follicular fluid and blood serum samples were analyzed for hormonal concentrations using commercial kits.
 The results showed that  the concentrations of estrogen,  progesterone and testosterone  in blood serum at season (54.31±0.49, 20.75 ±0.12, 0.75±0.05) respectively. While the concentrations of estrogen,  progesterone and testosterone in blood serum at out season (10.88±0.39, 0. 61±0.036, 0.063±0.042) respectively.The concentrations of estrogen,  progesterone and testosterone in follicular fluid at season (69.9±0.44, 28.46±0.82, 0.331±0.65) respectively. While  the concentrations of estrogen,  progesterone and testosterone in follicular fluid out season (there are no found ovarian follicles).    
 The present study a significantly higher (P< 0.05) in blood serum and follicular fluid  at  season than  that out season blood serum.