The objectives of this study were to isolate different bacterial species from goat urine, and determining some physical and chemical parameters in goat’s urine to record some microscopic changes in the urine sediments of the samples. Altogether, 200 urine samples were collected from 1-7 years old goats of both sexes from slaughtered goats in the abattoir in Mosul City, Iraq, half of them from male goats (bucks) and the other half from female goats (does).The number of control group was 88 samples in males and 80 samples in females.The results showed that the urine samples of infected animals were highly turbid, dark yellow color with low specific gravity. The microscopic examination of the urine sediments revealed increased number of pus cells (WBCs), erythrocytes, epithelial cells, casts and crystals/high power field (HPF) in the urine samples of infected animals in comparison with the urine samples of non-infected animals (free from bacterial isolates).The chemical examination of the urine samples revealed elevated urobilinogen, glucose, ketones, blood, proteins, leukocytes and pH levels of the urine samples of the infected animals with bacterial infections. Culture of urine samples revealed that the overall bacterial infection was 32 (16%) out of 200 urine samples.The infection rate with Corynebacterium renale was 2%, Corynebacterium cystitidis 2% , Corynebacterium pilosum 1.5% , Escherichia coli 2%, Staphylococcus aureus 2%, Streptococcus pyogenes 1.5%, Klebsiella pneumonae 1%, Enterococcus faecalis 1%, Pseudomonus aeruginosa 1%, Trueperella pyogenes1% and Proteus mirabilis 1%. The infection rate with different bacterial species was higher in female goats (Doe goats) than the male goats (bucks) and the infection rate was higher in old animals compared to the young animals.