Basrah Journal of Veterinary Research

EVALUATION OF THE ISOLATION AND DETECTION METHODS FOR SALMONELLA SPP. FROM EGG SHELL CONTAMINATION USING MULTIPLEX PCR.

Authors

Israa Adnan Ibraheam Al-Baghdady
Ashwak Bassim Jassim
Zainab Khudher Ahmed
https://doi.org/10.33762/bvetr.2015.102445
Abstract
A total of seventy four egg samples were collected from different markets sources, to
investigated for Salmonellaspp. the bacteria was isolated and identified using culturing on
selective media, in addition to, biochemical and serotyping by monovalent antisera.
Multiplex Polymerase Chain Reaction(PCR) detection of theinvA; and DH genes was
used for conformation of the identification of the Salmonella spp.. Twenty eight samples
from seventy four eggs provide positive results with PCR as Salmonella spp. depending
on InvA gene which is the target for the identification at the genus level.

Keywords

Egg Shell
invAgene
Multiplex PCR
Basrah Journal of Veterinary Research
Volume 14, Issue 1
Spring 2015
Page 282-288

  • Receive Date 01 December 2015
  • Revise Date 20 December 2015
  • Accept Date 25 December 2015
  • Publish Date 01 June 2015

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