INVESTIGATION OF INFECTIOUS LARYNGEOTRACHEITIS VIRUS IN IRAQI CHICKEN FARMS

Authors

Abstract
Twenty samples of Laryngeal and Tracheal tissues from laying chickens of two
flocks with suspicious infectious laryngeotracheitis were tested for the detection of the
causative virus by using virus isolation in chicken embryo fibroblast (CEF)cell culture
and chicken embryos by chorioallantoic membrane inoculation and conventional
polymerase chain reaction(PCR).The virus was isolated from three samples (larynx and
trachea) out of ten samples at Al-Sawara city on (CEF)cell culture and produce pock
lesions on chorioallantoic membrane of infected chicken embryos in which the virus was
isolated.
Positive PCR results were detected in the three clinical samples, isolated virus in
CEF cell culture and isolated virus in chicken embryos (inoculated on chorioallantoic
membrane).

Keywords


Volume 14, Issue 1
Spring 2015
Page 302-310

  • Receive Date 01 December 2015
  • Revise Date 20 December 2015
  • Accept Date 25 December 2015
  • Publish Date 01 June 2015