Authors
Abstract
Twenty samples of Laryngeal and Tracheal tissues from laying chickens of two
flocks with suspicious infectious laryngeotracheitis were tested for the detection of the
causative virus by using virus isolation in chicken embryo fibroblast (CEF)cell culture
and chicken embryos by chorioallantoic membrane inoculation and conventional
polymerase chain reaction(PCR).The virus was isolated from three samples (larynx and
trachea) out of ten samples at Al-Sawara city on (CEF)cell culture and produce pock
lesions on chorioallantoic membrane of infected chicken embryos in which the virus was
isolated.
Positive PCR results were detected in the three clinical samples, isolated virus in
CEF cell culture and isolated virus in chicken embryos (inoculated on chorioallantoic
membrane).
Keywords
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