The current study was conducted on extracted Azadirachtaindicaneem seeds oil using the ethanol, it was measured effectiveness inhibitory of neem seeds oil extraction in different concentrations of 0.001 and 0.003 unit/ml compared with commercial neem oil, known (Azadirachtin) concentration of 0.002 unit/ml on the Hayalomma Spp. The study was conducted on six calves were selected randomly with different ages ranging from 4-6 months in the veterinary clinic in Baghdad / Diyala bridge area. The animals were divided into three group with 2 animals each, namely control group that treated with 0.002 neem oil (Azadirachtin 3.000 ppm) and treatment groups 1 and 2 were treated with neem oil with concentration of 0.0001 and 0.0003 respectively. The parasitic infestation of Hayalomma Spp was confirmed in the laboratory diagnosis and the presence of ticks in addition to the clinical symptoms of infected calves.The objective of this study was to evaluate the effects of different concentrations of seeds oil of AzadirachtaIndica(Neem) on Hyalomma Spp eliminate on the Cows. Results showed high efficiency of neem oil concentration 0.003 unit/ml as an anti-ticks, where there was a decline of the number of parasites since of the first day to the fourth day of the treatment that showed the mean number of parasites isolated 18.0, 15.8, 3.5 and 0.0respectively, compared with concentration of 0.001 and control group with significantly reduced of the parasites number P< 0.004at 0.05. Also, the most important result showed that there was no side effects of the high concertation of 0.003 of neem oil to treat the infected animals. In conclusion, the concentration 0.003 of neem showed the best extraction to eliminate the parasites without effects of animals’health status.