Basrah Journal of Veterinary Research

ISOLATION AND IDENTIFICATION OF ALOCALSTRAIN OF PROBIOTIC BACTERIAL ACTOBACILLUS PLANTARUM AND STUDIED THE TOLERANCE ABILITY FOR DIFFERENT LEVELS OF pH

Authors

Sinan J. Abdul-Abbas
Adnan E. Al-Badran
Hasan R. Al-Sherifi
Aswan H. Aboud Al-bayyar
https://doi.org/10.33762/bvetr.2016.124330
Abstract
Lactobacillus plantarum IRQ12 was isolated from local pickle producing from Cucumis sativus submerged in 5% salt solution for one week far from sun light, selective media was used for isolation and activation process which was MRS broth and MRS agar, biochemical tests like catalase, nitrate reducing, productive gas, citrate consumption, produced ammonia from arginine, produced indole from tryptophan, fluid gelatin, ability of growth in 15, 45 °C, carbohydrates fermentation test, were carried out. DNA of bacteria was extracted by DNA extraction kit, identification by 16S rDNA gene. the local strain tolerance ability was investigated for different levels of pH 4, 3, 2 and 1.5 for 0, 1, 2 and 3 hours, log cfu/ml at pH 4 after 3 hours of treatment was 7.83 and at pH 3 the log cfu/ml decrease to 6.8 after 3 hours of treatment. The log cfu/ml was decreased to 5.76 and 5.05 at pH 2 and 1.5 respectively after 3 hours of treatment.

Keywords

Probiotic bacteria
plantarum
DNA extraction

Article Title العربیة

عزل وتشخيص البكتريا العلاجية Lactobacillus plantarum IRQ12 من المخلل المحلي ودراسة قابلية تحملها لمستويات مختلفة من الرقم الهيدروجيني

Basrah Journal of Veterinary Research
Volume 15, Issue 2
Autumn 2016
Page 329-345

  • Receive Date 01 December 2016
  • Revise Date 20 December 2016
  • Accept Date 25 December 2016
  • Publish Date 28 December 2016

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