Basrah Journal of Veterinary Research

ISOLATION, IDENTIFICATION OF SOME FUGAL ISOLATES AND TESTING THEIR ABILITY FOR LIPOXYGENASE PRODUCTION

Authors

Amal K. G. AL-Asady
Alaa G.E. AL-Hashimi
Nora T.Y. AL-Temime
https://doi.org/10.33762/bvetr.2017.127442
Abstract
Eighty three local isolates of fungi were isolated from different resources
(Peanuts , maize, rice, wheat, bread , domestic cheese of sheep, Milk local Cream,
Iranian cream, Roquefort cheese and soil). These isolates were purified and
identified, it include 14 isolates of Aspergillus flavus, 13 Aspergillus niger,8
Aspergillus terreus,3Aspergillus parasaticus,3Alternaria
spp.,15Penicilliumspp.,7Fusarium spp.,5 Trichoderma spp., 11 Rhizopus spp. and
7Mucor spp. The ability of isolates for producing aflatoxin were tested, the toxic
isolates(Aspergillus flavus,Aspergillus terreus ,and Aspergillus parasaticus) were
removed.Aspergillus niger which was isolated from maize was choosing as the best
lipoxygenase producer after Primary and secondary screening. The growth of the
selected isolate colonies had the largest proportion than the Colonies of Penicillium
sp. and Trichoderma sp..all so the same isolate had high enzymatic activity
801.4units/ml, while Penicillium sp. and Trichoderma sp. had (559.2 and 120)
units/ml respectively.

Keywords

Fungi
Aspergillus niger
Lipoxygenase
Basrah Journal of Veterinary Research
Volume 16, Issue 1
Spring 2017
Page 120-135

  • Receive Date 01 June 2017
  • Revise Date 20 June 2017
  • Accept Date 25 June 2017
  • Publish Date 28 June 2017

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