One of an ecological hazardous agent Chromium; Cr (VI), The present experiment
in vitro design to examine the mechanism of Leydig cell functions of Cr (VI) in ram
testis, Cr (VI) treated groups demising cell growth behavior of exponential phase was
upset of feeding time to Leydig cell in a dose-dependent manner, and induced
mitochondria-dependent ATP depletion and subsequently apoptosis. Cr (VI) effect
may be attributed, at least in part to DNA fragmentation increase DNA tail number
and tail length of COMET as compared with control group. Furthermore, the
properties of cell-specific regulation of cell membrane integrity had reduced and
determinant cell concentration drop an.d reflected on the testosterone concentration
were decreased as concentration-dependent manner, In conclusion, our results display
the Cr (VI) is cytotoxic and impairs both viability and steroidogenic functions of
Leydig cells in ram testis via actually different pathway direct affecting of viability
and indirect on steroidogenic activity, succeeding in testicular performance. However,
the definite modes of action of harmfulness are not evidently unknown and must be
rechecked and studied in a different aspect