Basrah Journal of Veterinary Research

DETECTION OF BACILLUS THURINGIENSIS CRYSTAL PROTEIN CRY51AA1 INTOXICATION (IN VIVO) USING ZEBRAFISH MODEL

Author

Ali B.T. Aldeewan
https://doi.org/10.33762/bvetr.2017.143529
Abstract
Bacillus thuringiensis is a ubiquitous a gram-positive, anaerobic, spore-forming
bacterium, produces various proteins (toxins) during the stationary and vegetative phase
of its growth cycle. Some of these proteins belonging to this group showed weak
similarities to ß-barrel pore-forming toxic proteins, such as the cytotoxins of
Pseudomonas aeruginosa, the epsilon toxin of Clostridium perfringens, alpha-toxin of
Clostridium septicum. In the present study, the intoxication effects of Cry51Aa1 protein
produced by B.thuringiensis F14-1 strain was evaluated in vivo, in order to investigate the
ability of activated Cry51Aa1 to make a change in size or functions of zebrafish
cardiovascular system and induces toxicity in adult zebrafish. Using in vivo imaging we
observed that Cry51Aa1 has no scientific effect on cardiac function and development of
zebra fish embryo or have toxic effect on adult in spite of use high concentration, so it is
safe to use and a potentially effective agent in breast cancer therapy.

Keywords

Bacillus thuringiensis
Toxins
in Vivo
Basrah Journal of Veterinary Research
Volume 16, Issue 2
Autumn 2017
Page 26-35

  • Receive Date 01 December 2017
  • Revise Date 20 December 2017
  • Accept Date 25 December 2017
  • Publish Date 28 December 2017

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