Author
Abstract
Retinitis is the most important clinical consequences of Toxoplasmosis but the
mechanisms used by T. gondii tachyzoites to invade the retina are not yet understood. In
the current study, cellular pathogenicity of Toxoplasmosis in a human retinal pigment
epithelial cell line (ARPE-19) was investigated. Following in vitro infection of ARPE-19
with T. gondii tachyzoites, cell viability associated with infection was identified by
Methyl thiazolyl tetrazolium (MTT) assay. Intracellular development of T. gondii
tachyzoites within ARPE-19 was characterized by acridine orange (AO) staining.
Morphological changes associated with infection was assessed by electron microscopy
(EM). The results obtained showed that ARPE-19 permit the invasion, growth and
development of T. gondii tachyzoites and that infection can cause a metabolic burden on
the host cells and multiple morphological changes in the relocation of the host cell
organelles (mitochondria, endoplasmic reticulum, and Golgi apparatus) around the
parasitphorous vacuole
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