The present study aims to evaluate the histopathological changes induced by recombinant
follicle stimulating hormone (follitropin alpha) on reproductive organs as well as the liver and
kidneys of female rats. The experiment was done on 24 white female rats (Rattus norvegicus)
sexually mature weighing 150-200 gram, divided into 4 equal groups of 6 animals: control group
which was given distilled water. Single dose group, double dose group and triple dose groups
which were injected by 0.5 iu.,1 iu. and 1.5 iu of recombinant FSH respectively. The drug was
given subcutaneously during the pro-estrous phase for ten consecutive cycles, then animals from
each group were sacrificed to study the histopathological changes. The histopathological
examination of the ovaries, uterus, liver, and kidneys revealed variable changes in different
organs.The ovarian sections showed many Graafian follicles without ova and many corpus luteal
cysts, fibrosis, and thickened granulosa cell layer, and the ovary was surrounded by excessive
adipose tissue. The uterus in single and double doses showed dilated cavity, thin endometrium,
thin muscular layer and diminished endometrial glands while in triple dose showed atrophy of
endometrial lining and glands, hypertrophied muscular layer with slit like endometrial cavity and
formation of multiple endometrial cyst. The liver sections showed few changes like dilated
central vein, congestion of sinusoids, vacuolation of hepatocytes, with moderate degree of fatty
degeneration A few hepatocytes appeared necrotic but without inflammatory response. The
kidneys in single and double doses showed unremarkable changes, while in triple dose
glomerular congestion, congested vessels, hemorrhage, and degeneration and necrosis of
proximal tubules were found.