Basrah Journal of Veterinary Research

GENETIC POLYMORPHISM AND DIVERSITY OF IRAQI AWASSI SHEEP USING PCR-RAPD TECHNIQUE

Document Type : Research Paper

Authors

Zainab S. Al-Allak 1
Maytham A. Dragh 2
Ahmed Sadoon Hussain 3

1 Department of Animal Production, College of Agriculture, University of Misan, Misan, Iraq.

2 Department of Biology, College of Science, University of Misan, Misan, Iraq

3 Department of Animal Production, College of Agriculture, University of Misan, Misan, Iraq

https://doi.org/10.23975/bjvetr.2020.170619
Abstract
The establishment of modern sheep production systems in Iraq, lead to presence of various
forms of hybridization between the native and Middle East breeds which have been utilized for
genetic improvement. This occur in consistence with the progressive destruction or deterioration
of sheep habitat. Together, these factors have accelerated the loss of genetic diversity or even
resulted in the extinction of some indigenous breeds. Therefore, it is important to develop
efficient strategies for surveillance, evaluation, conservation and utilization of the available
genetic resources for this species. Seven random amplification polymorphism DNA (RAPD)
marker used. The aim of this study was to assess genetic diversity for Awassi native breed in
Iraq. The higher polymorphism information contents at the seven markers (Seventy- three bands
obtained with 28.3% of polymorphism) indicate the retention of natural variation from source
populations for the domestic breeds of different geographic regions in Iraq. Analysis of genetic
differentiation revealed substantial divergence among these breeds as 16% diversity indicating
that some evolutionary forces (e.g. selection and migration, uncontrolled selling across borders)
had acted on these populations. Phylogenetic and phylogeographic analyses displayed a
remarkable degree of consistency between geographic origins, breeding histories and the pattern
of genetic differentiation.

Keywords

Awassi
polymorphism
PCR-RAPD

Article Title العربیة

عنوان البحث بالعربی)التشکل والتنوع الوراثی فی اغنام العواسی العراقیة باستخدام تقنیة-PCR RAPD

Abstract العربیة

ان انشاء أنظمة تربیة الأغنام الحدیثة أدى الى وجود اشکال مختلفة من التھجین بین السلالات المحلیة وسلالات الشرق
الأوسط والتی خضعت للتحسین الوراثی وھذا حدث بالتزامن مع التحویر والتدمیر المستمر لبیئتھا الطبیعیة وبالتالی فان ھذه
العوامل أدت الى فقدان التنوع الوراثی ولذلک أصبح من الضروری تطویر استراتیجیات کفؤة للمحافظة على المصادر الوراثیة
لھذه الحیوانات. استعمل فی ھذه الدراسة سبعة بادئات للتضخیم العشوائی للحامض النووی منقوص الاوکسجین -PCR
. RAPD markersالھدف من ھذه الدراسة کان تقییم التنوع الوراثی لسلالة العواسی المحلیة العراقیة واظھرت نتائج ھذه
الدراسة ان ھنالک تنوعا وراثیا وصل الى ) %٢٨.٣أی ما یعادل ٧٣حزمة تضخیم( وھذا یثبت تراجع التباین الطبیعی
للمناطق الجغرافیة المختلفة فی العراق. ان تحلیل التباین الوراثی اظھر اختلافا ثابتا ومتشعبا" بین ھذه السلالات وصل الى
%١٦مما یعنی ان بعض القوى مثل الانتخاب والھجرة والبیع غیر المسیطر علیھ عبر الحدود اثرت کثیرا" على ھذه السلالات
ان التحلیل الوراثی والجغرافی اظھر درجة عالیة من التباین بین سلالات العواسی المحلیة

Basrah Journal of Veterinary Research
Volume 19, Issue 1
Spring 2020
Page 147-154

  • Receive Date 02 September 2019
  • Revise Date 01 January 1970
  • Accept Date 30 October 2019
  • Publish Date 01 June 2020

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