The study was conducted to add information to the database for concentrate on the
Brunner's glands of the duodenum in adult local cows. The histological study showed
that Brunner's glands present in the tunica submucosa of the duodenum in local adult
cows, the glands were branched tubuloalveolar secreting mucous, extended from the
end of the pylorus to the first and middle third of duodenum, and absent at the last
third of the duodenum. The excretory ducts of Brunner's glands opened at the crypts
of lieberkuhn at the base of intestinal villi penetrating the muscularis mucosa layer.
The statistical analysis showed there was a significant variation in the diameter and
lumen of Brunner's glands between the first and middle third of duodenum at (P≤
0.05). The diameter and lumen of Brunner's glands at the first third of duodenum were
lower than that of the middle third of duodenum. The statistical study showed , that
there was no significant variation of the lining epithelium of Brunner's glands between
first and middle third of duodenum, and there was no significant variation in the
thickness of Brunner's glands aggregation between first and middle third of the
duodenum.
The histochemical study revealed that there were different types of glycoprotein in
Brunner's glands and excretory duct in local adult cows, at first third of duodenum
Brunner's glands contain a large amount of neutral glycoprotein and little amount of
acidic glycoprotein, while at middle third of duodenum the Brunner's glands contain
the same amount of neutral and acidic glycoprotein. At the first third of duodenum,
the excretory ducts of Brunner's glands contain neutral glycoprotein more than acidic
glycoprotein, while at the middle third of duodenum the excretory ducts of Brunner's
 glands contain a large amount of acidic glycoprotein and little amount of neutral
glycoprotein.