This study was conducted to determine the type of blood hemoglobin blood and its effect on biochemical parameters, which include the lipid profile and hormones of local cows in Basrah  province  by obtaining blood samples for cows from different areas of Basrah province in order to determine the type of hemoglobin and its effect on hormones and lipid parameters. Results show that hemoglobin type (B) achieved highest percentage of two types (AB and A) respectively. A significant elevation hemoglobin type (B) with the highest level of total cholesterol and triglycerides. The lipoprotein results (LDL-C, HDL-C, and VLDL-C) showed in hemoglobin type (B) was superior to that of another hemoglobin which having serum lipoprotein levels in lactation and pregnancy periods. Different hemoglobin phenotypes showed elevation of progesterone and estrogen levels in some physiological periods while hemoglobin type (B) can be seen to have a higher level in control periods and pregnancy periods when compared with other hemoglobin types. As for cortisol, it showed increase for hemoglobin type (B), while the lowest level of hemoglobin type (AB) when compared with physiological conditions (control, estrus, pregnancy, and lactation). It can concluded  that type of hemoglobin effect on sex hormones and lipoproteins that using them as indicators in improving economic production and choosing the good type of hemoglobin in breeding, improving and increasing animal production and milk production.