In recent years with the widespread use of medication, the relevance of drug nutrient interactions in daily practice continues to increase.Such interactions can involve one or more nutrients, present in food and nutrition. Drug food interactions can occur mechanistically in the absorption, distribution, metabolism and excretion of intestinsas well as in the interaction of receptors with drugs. Accordingly, in an in vivo rat study, the study assesses the effect of long term use of high and low doses of nimodipine on serum levels (Zn, Cu and Fe).,Twenty four male Sprague Dawly rats were assigned to three groups of 8 rats each, the first group was treated with D.W as a control group, the second group was treated with 20mg / kg nimodipine and the third group was treated with 80mg / kg nimodipine, all groups received oral treatment for 30 days, On day 31 all groups of rats were sacrificed and blood samples were drawn and collected for later analysis of trace elements in the polyethylene tubes. The findings showed that nimodipine treatment cause a significant decrease Concentration of the serum iron and zinc compared with control with lowest serum iron level observed in group received high dose of nimodipine. However, compared with control, both low and high doses of nimodipine had no effect on serum copper concentration. In conclusion using low or high doses of nimodipine lead to significant decrease in serum concentration of iron and zinc but no effect was observed in the serum concentration of copper compared to the control group level.