Document Type : Research Paper
Author
Department of Microbiology, College of Veterinary Medicine, University of Basrah, Iraq
Abstract
This study was conducted to elevated the ethanol and methanol of Salixa acmophylla
extraction on bacteria Staphylococcus aureus isolated from skin wound of cattle, about
100(66.66%)from 150 sample isolated , and made the biochemical test , also sensitivity of
antibiotic .The loaded antibiotic discs namely: gentamicin (CN-10 µg), levofloxacin
(LEV -5µg), amoxicillin (Ax -25 µg) , tetracycline (TE -30 µg)
,vancomycin(30µg),erythromycin (25µg)and streptomycin(25 µg) were placed on the
surface of the medium and left for 30 min at room temperature for complete diffusion. The
plates were incubated for 24 hrs. at 37°C. also made antimicrobial against isolated
bacteria disk diffusion techniques , Minimum inhibition concentration (MIC) with different
concentration of plant extracts (25mg /ml ,50 mg/ml, 100 mg/ml, 150 mg/ml, 200 mg /ml
and 300, . Minimum bacterial concentration (MBC) by Macro-dilution broth method were
done . Anti-inflammation test albumin denaturation Maximum inhibition in case of
ethanol extract about (65.25±1.2 %) and in methanol extract about (58.135±1.23) was
observed at concentration 300 µg/ml., Aspirin, a standard anti-inflammatory drug showed
the maximum inhibition ، (67.35±0.56%) at the concentration of (100µg/ml )
Keywords
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