Document Type : Research Paper
Author
Abstract
Lumpy skin disease (LSD) is a contagious viral infection of cattle caused by lumpy
skin disease virus (LSDV). This infection has a huge economic impact on the cattle industry
as a result of skin damage, inflammation of mammary glands and decrease milk production,
reduces reproduction and even death as a consequence of secondary bacterial infection. Iraq
is considered as an endemic country by OIE that continues breaking throughout different
parts of the country. The aims of this research work was to detect and molecular
characterization of LSDV in Duhok province for the first time through using a phylogenetic
approach of P32 and RPO30 genes. The results showed that LSDV was detected and
identified over using PCR and sequencing and the isolate 1 LSDV/Duhok/Kurdistan/2019,
isolate 2 LSDV/Duhok/Kurdistan/2019 and isolate 3 LSDV/Duhok/Kurdistan/2019 were
clustered in LSDV lineage for both P32 and RPO30 genes. The phylogenetic analysis of
these isolates showed a close relationship with the previously published isolates based on P32
gene sequences, while this study is the first study to publish genetic information on RPO30
gene of the Iraqi isolates and compare them to the other publicly available data sets of other
countries. To sum up, the findings of this paper could be beneficial to illustrate the spreading
nature of LSDV in Iraq and mainly in the Kurdistan region of Iraq. Furthermore, offer the
theoretical references for control and prevention of LSDV infections in future.
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