Document Type : Research Paper
Abstract
The isolate of Alternaria alternata that isolated from soil produced extracellular
secondary metabolites (Mycotoxin) of carbohydrate with reducing nature which had
inhibitory effect against Gm positive & Gm negative standard and pathogenic bacteria in
solid media. Three types of media Malt Extract Agar (MEA), Potato Dextrose Agar
(PDA) and Potato Carrot Agar (PCA) can be using as solid or liquid media for production
this mycotoxin. The mycotoxin extracted from free cell supernatant by simple extraction
method using ethanol 90%. The cytotoxicity of extracted mycotoxin against human blood
cells (RBCs) was (25) ppm. The acute toxicity of extracted toxin as determined by LD50
of laboratory mice was 150 mg/kg.
Keywords
)
