The aim was to investigate the effect of glycerin and whole egg powder supplements in the diet on the growth performance of Japanese quail. The current study included two experimental periods. 90 birds were used in the first experiment (digestive balance experiment), which lasted for one week, and 150 birds in the second experiment (growth and production), which lasted for 42 days. The treatments in the first experiment were as follows: T1: Control, T2: Glycerin 2.5% g/kg feed, T3: Whole egg powder 2.5% g/kg feed. While 150 chicks were used in the second experiment (production period) randomly distributed into 5 treatments: T1: Fasting(24h), T2: Control, T3: Glycerin (2.5%), T4: Whole egg powder (2.5%), and T5: Glycerin (2.5%) with whole egg powder (2.5%). The results of this study showed the highest significant difference (P ≤ 0.05) was found in the daily and weekly average live body weight, daily, weekly and cumulative weight gain, feed consumption and feed conversion ratio, apparent and true metabolic energy, lengths of parts of the digestive tract (liver, proventriculus, duodenum, jujenum, ilum, cecum and rectum), Apparent metabolizable energy, protein digestibility factor in the treatment of birds treated with a 2.5% g/kg glycerin supplement compared to the control. Finally, this study concluded that the use of glycerin as a nutritional supplement effectively contributes to improving growth performance of Japanese quail used as an alternative source of poultry meat.