Due to a lack of information about asymptomatic toxoplasmosis(A.T.) and its prevalence in cats and their owners in Basrah city. Study was established to assess the seroprevalence of toxoplasmosis and associated factors among cats and adopted households . The study's results showed that cats and their owners had high levels of (A.T.). The female cats are significantly more vulnerable to infection by toxoplasmosis compared to male cats. In the case of feline toxoplasmosis and age-related, the cats can be infected at any age, and the junior cats are more susceptible to having toxoplasmosis. Studying feline toxoplasmosis and its relationship to lifestyle revealed that animals raised and living outdoors had a higher infection rate. The feeding style plays a significant role in feline toxoplasmosis, and it is increased when the animals feed on 100% home leftover food. The animals that had a good veterinary health coverage are significantly less vulnerable to having (A.T.). The breed of animals does not affect the rate of infection. The cat owners had high levels of (A.T.). No significant effect on the prevalence of infection in humans regarding age and sex , indicating that all genders and ages are susceptible to infection. However, the lifestyle and the level of veterinary health knowledge of the pet owner had a significant effect. So , the (A.T.) represented a serious threat to animals' and humans' health. Using the rotten examination sported with treatment , a proper methods to management and treated animals are essential for prevention.