Ovarian physiology is controlled by many exogenous and endogenous factors including the endocrinological and biochemical alteration that occur in the follicular fluid during the estrus cycle. the aim of this study was to determine and compare the concentrations of some hormonal and biochemical composition in peripheral circulation and follicular fluid of buffaloes .For this purpose ,ovaries collected from adult buffaloes immediately after slaughter, and blood samples where also collected from these buffaloes before slaughter in Basra abattoir.
Samples were classified into two groups according to the follicle diameter, small (4-8 mm) and large (> 8 mm). The follicular fluid and plasma samples were analyzed for biochemical composition using commercial kits.
The results showed that small follicles had significant higher (p<0.05) , T3, glucose, and cholesterol concentrations than large follicles while no differences were found in concentrations of TSH between both groups .The concentrations of ,T4, progesterone, estrogen, and total protein were significantly higher (P< 0.05) in large follicles than small follicles.
The results of the present study also indicated that the TSH, T4, glucose, total protein and cholesterol concentrations in the blood plasma were significantly higher