Basrah Journal of Veterinary Research

EFFECT OF IMMUNOGLOBULIN Y PURIFIED FROM IMMUNIZED HEN EGGS ON THE GROWTH OF STAPHYLOCOCCUS AUREUS

Author

Ali A. Al-Edany
https://doi.org/10.33762/bvetr.2011.55030
Abstract
A novel purification method of egg yolk immunoglobulin (IgY) based on
precipitation using agar-PEG was developed. This method was compared with
chloroform extraction and polyethylene glycol (PEG) precipitation methods. The
results showed the protein contents were high with chloroform method followed by
agar-PEG then PEG method. The purity of resultant IgY was homogeneous with agar-
PEG method followed by PEG method then chloroform extraction method. The IgY
purified by agar –PEG method, obtained from hens immunized by formalin-treated S.
aureus, showed a significant reduction in bacterial growth and the growth inhibition
was dependent on specific-IgY concentration

Keywords

IgY
chloroform
aureus

Article Title العربیة

تاثي الجلوبين المناعيY المنقى من بيض الدجاج الممنع على المكورات العنقوديه

Author العربیة

علي عبود العيداني
Abstract العربیة

تم تطوير طريقةلتنقية الجلوبين الماعي Yمن صفار البيض وهي مبنية على اساس الترسيب باستخدام الاجار-متعدد الاثيلين جلايكول تم مقارنة هذه الطريقةبطريقة استخلاص الكلوروفوموطريقة الترسيب بمتعددالاثيل جلايكول

Basrah Journal of Veterinary Research
Volume 10, Issue 2
Autumn 2011
Page 85-96

  • Receive Date 01 December 2011
  • Revise Date 20 December 2011
  • Accept Date 25 December 2011
  • Publish Date 28 December 2011

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