In the present study, Low density polyethylene with various contents of lignin films were investigated for their resistance to bacterial adhesion. The disc diffusion method was employed for this purpose. The results of bacterial growth on low-density polyethylene (LDPE )–lignin composite were reported. lignin was added by 1%,2%,3%,4%,5%,10% and 15% of polyethylene weight. We compared initial adhesion and surface growth of staphylococcus aureus , streptococcus pyogens, pseudomonus aerugenosa, eschrishia coli and klebciala spp. A 5 mm of test polymer composites were inoculated in the 9 cm Petri dish for 1-2 days and zone of inhibition and bacterial growing were observed and recorded. After 24 hours the bacterial growing of S. aureus , S. pyogens and E. coli were distributed all over the control agar with no growth on the polymeric composites films while Both P. aeruginosa and K. spp. have a dense growing. After 48 hours a slight adhesion of S. aureus on polyethylene-lignin discs was found with radius of growing was 3 mm .For P. aeruginosa we have more dense growing . It was found that the lignin contents have no clear inhibition against tested bacteria where this might be due to a limitation of the agar disc diffusion method. The vanishing of inhibition zone was combined with the absence of adherent bacteria on the polyethylene films. More concentration of natural lignin is required to get inhibition effect.