Bacteriological examination was performed on 60 local healthy fish of fresh water include 30 Carp fish (Cyprinus carpio) and 30 Cat fish (Silurus glanis) with different weights from local retail fish markets at Mosul city, during the period from sept. 2011 - Sept. 2012. Swabs from skin and parts of muscles, livers, intestines incubated in brain heart infusion broth for 24 hours at 37 ̊ C (aerobic culture), a loopful from incubated broth were streaked on blood agar, milk agar, mannitol salt agar incubated plates at 37 ̊ C for 24 h, selected colonies were submitted to gram staining, morphological characteristics biochemical tests for Staphylococcus. The percentage of Staphylococcus isolation was 100% for all examined samples of fish. A total of 130 isolates from both two species of examined fish (62) isolates from Cyprinus carpio and (68) isolates from Silurus glanis , a five species of Staphylococcus S. saprophyticus (29% , 29.4%), S. epidermidis (21% , 22%), S. hyicus (17.75% , 17.7%), S. aureus (17.75% , 19.1), S. intermedius (14.5% , 11.8%) , were identified with different numbers and percentages for Cyprinus carpio and Silurus glanis respectively . While percentages of Staphylococcus isolates from skins (35.5% , 36.8%), muscles (17.7% , 20.6%), livers (25.8% , 25%), intestines (21% , 17.60%) from both species Cyprinus carpio and Silurus glanis respectively. Antibiotic sensitivity test result for six antibiotics (Ampicillin, Gentamicin, Chloramphenicol, Polymaxin, CO-Trimaxazol , Ciprofloxacin) were variable most species of Staphylococcus isolates were resistant to Ampicillin but sensitive to Ciprofloxacin.