This study was designed to find out the effect of oral inoculation of aqueous extract of Curcuma Longa at two doses (1 and 5) mg/ kg body weight daily for 4 weeks on the immune response of Balb/c mice by estimating of serum concentration of interleukin-2 (IL-2) , interleukin-4 (IL-4), interleukin-10 (IL-10) and interferon gamma (INF-γ) using ELISA test. The present results revealed that significant increase (p<0.05) in the values of both of IL-2 (133.80 pg/ml and 181.60 pg/ml, respectively) and INF-γ concentration (789.50 pg/ml and 1131.50 pg/ml, respectively) in sera of both mice groups treated with two concentration of Curcuma longa 1and 5 mg/kg body weight, respectively in comparison with control group. On the other hand significant elevation of IL-4 (91.00 pg/ml and 64.40 pg/ml, respectively) and IL-10 concentration (50.10 pg/ml and 42.70 pg/ml, respectively) in sera of both mice groups treated with Curcuma longa 1 and 5 mg/kg body weight in comparison with control group. IL-2 and INF-γ were used for detection of TH1 response, while IL-4 and IL-10 used for TH2 response detection. However, both mice groups treated with Curcuma longa (1 and 5 mg/kg) showed increase in the activity of TH1 in comparison with TH2. The ratio of IL-2/IL-10 (4.253) for mice group treated with 5 mg/kg body weight Curcuma longa, and INF-γ/IL-4 (17.659), and these rates were higher than the ratio of IL T 2/IL-10 (2.671) and INF-γ/IL-4 (8.676) for mice group treated with 1 mg/kg body weight