USING TWO DIFFERENT PROMOTERS OF LYSOSTAPHIN GENE TO CHANGE PROTEIN PRODUCTION

Author

Abstract
Bacteriocins are bacterial antimicrobial peptides with bactericidal activity
against other bacteria. Staphylococcins are bacteriocins produced by staphylococci,
which are gram-positive bacteria with medical and veterinary importance. In this
study we are using two promoters from two plasmids to lysostaphin gene to change
production of lysostaphin protein then killed Staphylococcus aureus and compared
with pBluescript plasmid as control, pTrc99a get increased the production of
lysostaphin protein while the pBAD30 are decreased the production in compared with
pBluescript plasmid as control, at the same time both plasmid reported succeeded to
lysostaphin expression and gave the protein with different concentration.

Keywords


Volume 13, Issue 2
Autumn 2014
Page 141-150

  • Receive Date 01 December 2014
  • Revise Date 20 December 2014
  • Accept Date 25 December 2014
  • Publish Date 28 December 2014