Authors
Abstract
In order to determine the genotoxic effects of diazinon and the role of
chitosan to neutralize these effects, our study performed in (24) male rats
(Rattus norvegicus) were divided into four groups and treated for (60) days
as following, group (A) treated with normal saline and served as control,
group (B) treated with [(1/10LD50) 3.8mg/kg. bw] of diazinon, group (C)
treated with [(1/10LD50) 3.8mg/kg. bw] of diazinon and fed on diet
supplement containing (1gram/1kg ration) chitosan, group (D) fed on diet
supplement containing (1gram/1kg ration) chitosan only. The genotoxic
effect of diazinon was evaluated by using the micronucleus assay showed
increasing of micronucleated polychromatic erythrocytes were (11.6%) in
group B, while (7%) in group C . The chromosomal aberration showed
increase of presence of chromosomal aberration in group B was (7.5±1.04),
while in the group C showed mild elevation in (3.25±0.8). The polymorphism
of GSTM1 and GSTT1 genes showed highly incidence of both genes
polymorphism in group B was (66.6%) while group C was (50%) . we
concluded that diazinon is genotoxic pesticide and chitosan ameliorate it
effects.
Keywords
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