Authors
Abstract
Assessment of the sperm membrane functional status appears to be a significant
marker for the fertilizing capacity of spermatozoa. The hypo osmotic swelling test
(HOST) is one of the best methods to evaluate sperm membrane integrity. In the
current study, we used DW and hypo osmotic solutions of 50 and 100mOsm/l of
dextrose/NaCl, NaCl, sucrose and fructose. Based on the results, Among the
dextrose/NaCl, NaCl, sucrose, and fructose solutions and DW, Maximum numbers of
swollen of bull frozen-thawed spermatozoa were observed with DW and
dextrose/NaCl solution at 50 mOsm with average response by 61.20±8.677 and
47.90±10.181 respectively. The HOST response at 3 and 60 min for all of solutions
were positively correlated to each other and there was no significant difference
between the responses to the HOST at 3 and 60 min after incubation in all of
solutions. The significant correlation was observed between motility and
dextrose/NaCl at 50 mOsm, sucrose 50 and 100 mOsm, NaCl 50 mOsm and DW. The
high relationship was between motility and DW and dextrose/NaCl at 50 mosm. There
was no significant correlation between DW and all of hypoosmotic solutions with
staining of the spermatozoa by eosin/nigrosin. In conclusion, the water test can be
efficiently used for the evaluation of the functional integrity of the plasma membrane
of bull frozen-thawed spermatozoa. The hypoosmular solution of dextrose/NaCl at 50
mOsm is a good medium to evaluate bull frozen spermatozoa. The used of HOST and
motility are better tests to evaluate bull frozen thawed sperm than eosin-nigrosin. The
short HOST procedure (3 min) is suitable method for evaluating of membrane
integrity of bull frozen/thawed spermatozoa.
Keywords
Article Title [العربیة]
EVALUATION OF MEMBRANE INTEGRITY OF BULL FROZEN-THAWED SPERM USING WATER AND HYPO OSMOTIC SWELLING TEST
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