Basrah Journal of Veterinary Research

13- STUDY THE EFFECT OF DIFFERENT CULTURE MEDIA ON SOME FROZEN SEMEN CHARACTERISTICS OF IRAQI BLACK LOCAL GOAT SPERMATOZOA

Authors

Taher A.Fahad
Khairi G. Al-Rikabi
Abdulrazak N. Khudair
https://doi.org/10.33762/bvetr.2018.144947
Abstract
The semen was collected from five adult Iraqi black local bucks by the
artificial vagina and using estrous induced doe the semen was diluted with egg yolk
10% extender and frozen by liquid nitrogen for three months, macroscopic and
microscopic evaluation were done after collection, dilution and thawing, three
media(TCM-199, DMEM and SOF)were used to see the their effect on activation of
spermatozoa. The results showed that TCM-199 media presented higher significance
in sperm motility, progressive motility compared with DMEM and SOF media.
There was a great decrease in the concentration of spermatozoa after activation using
these media which didn’t differ significantly among them and they were higher
significantly compared with control in normal morphology.

Keywords

Bucks
DMEM
Sperm motility
Basrah Journal of Veterinary Research
Volume 17, Issue 1
Spring 2018
Page 147-154

  • Receive Date 01 December 2018
  • Revise Date 20 December 2018
  • Accept Date 25 December 2018
  • Publish Date 01 June 2018

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