study aimed to investigate the incidence of tnsA, tnsB, and tnsC and their correlation with antibiotic resistance profiles in E. coli isolated from human and dairy cattle in Basrah. According to PCR results the tnsA was the most prevalent element at 59%, followed by tnsC (34%) and tnsB (28%). Human isolates exhibited a higher frequency to (tnsA: 65%, tnsB: 41%, tnsC: 41%) compared to animal isolates (tnsA: 50%, tnsB: 8%, tnsC: 25%). The triple pattern (tnsA + tnsB + tnsC) representing a complete core machinery was found in 21% of all isolates, showing high prevalence in human sources (29.4%) contrasted with animal sources (8.3%). Furthermore, the antibiotic resistance profiling was correlated with seven distinct tns genotypic patterns: Triple (tnsA+B+C), Double (tnsA+B, tnsA+C), Single (tnsA, tnsB, tnsC), and None (Negative). The isolates with triple (tnsA+B+C) pattern were showed ahigh resistance to ampicillin (86%), azithromycin (67%), and cefotaxime(86%),ceftazidime (71%) and augmentin (50%), but lower resistance (17%) to aminoglycosides like gentamicin and amikacin. Whereas, double (tnsA+B) and Single (tnsB) configurations exhibited complete 100% resistance to azithromycin, cefotaxime, ceftazidime, augmentin, and ampicillin. The isolates with single (tnsA) were showed a high resistance to ampicillin (100%), cefotaxime (86%), and ceftazidime (71%), while single (tnsC) isolates displayed 100% resistance to tetracycline, cefotaxime, augmentin, and ampicillin. On the other hand, two negative associations were observed between the presence of the targeted transposons and antibiotic resistance; first: no chloramphenicol resistance was detected in isolates possessing these elements and second: resistance was identified in 40% of the transposon-negative isolates